Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells

STAR Protocols

Yuan Hu¹  Xiawei Wei²  Min Luo³  Kun-Liang Guan³ 

[1]Corresponding author

[2]Department of Pediatric Nephrology Nursing, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second Hospital, Sichuan University, Chengdu 610041, China

[3]Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, Cancer Center, West China Hospital, Sichuan University, No. 17, South of Renmin Road, Chengdu 610041, China

关键词: Cell Biology;    Cell culture;    Cell-based Assays;    Microscopy;    Signal Transduction;    Protein Biochemistry;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The Hippo pathway plays critical roles in cell growth, differentiation, development, tissue homeostasis, and cancer. Here, we describe protocols to measure phosphorylation, subcellular localization, and kinase activity for core Hippo pathway components YAP and LATS. The phos-tag technology is particularly useful to quantitatively detect protein phosphorylation even without a phosphospecific antibody. We use HEK293A cells as an example, but the protocols can be applied to other cell lines and tissues.For complete details on the use and execution of this profile, please refer to Luo et al. (2020).

【 授权许可】

Unknown