Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines

Journal of Functional Foods

Alex Galanis¹  Eleftheria Lamprianidou²  Ioannis Kotsianidis²  Athanasios Karapetsas³  Aikaterini Chlichlia³  Georgia Saxami³  Chrysoula Tassou⁴  Vassilis Zoumpourlis⁵ 

[1] Biotechnology, National Hellenic Research Foundation, Athens, 11635 Greece;Department of Hematology, Democritus University of Thrace, Medical School, Alexandroupolis 68100, Greece;Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece;Institute of Technology of Agricultural Products, Hellenic Agricultural Organization – DEMETER, Sof. Venizelou 1, Lycovrissi, Attiki, 14123 Greece;;Unit of Biomedical Applications, Institute of Biology, Medicinal Chemistry &

关键词: Probiotics;    Lactobacillus;    Adhesion;    Anti-proliferation;    Caco-2;    Conditioned media;   

DOI  :  

来源: DOAJ

【 摘 要 】

The beneficial effects of two potential probiotic lactobacillus strains, namely Lactobacillus pentosus B281 and L. plantarum B282 were examined and the mechanisms of action were investigated. Quantitative analysis and confocal microscopy showed that both strains exhibited a significant higher adherence to Caco-2 cells in comparison to the reference strain L. casei ATCC 393. Treatment with conditioned media (CM) of the two strains caused significant reduction of cell proliferation, as demonstrated by Sulforhodamine B (SRB) and clonogenic assays. Moreover, the CM of the two strains induced a G1-phase arrest and down-regulation of specific cyclin genes, as indicated by flow cytometry and real-time PCR analysis. To begin elucidating the nature of the bacterial components conveying these responses, the anti-proliferative effect of heat-treated CM was analysed. The anti-proliferative activity of heat-treated CM was similar to the non-heated CM in a time- and dose-dependent manner, indicating the presence of thermostable bioactive compounds.

【 授权许可】

Unknown