Probiotic Candidates from Fish Pond Water in Central Java Indonesia

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Haditomo, Alfabetian Harjuno Condro^1 ; Desrina^1 ; Sarjito^1 ; Prayitno, S. Budi^1

Department of Aquaculture, Fisheries and Marine Science Faculty, Diponegoro University, Tembalang, Semarang, Indonesia^1

关键词: 16s rDNA;    16S rDNA sequence;    Aeromonas hydrophila;    Bacillus subtilis strains;    Bacterial pathogens;    Laboratory experiments;    Pond water;    Probiotics;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012018/pdf
DOI  :  10.1088/1755-1315/116/1/012018

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Aeromonas hydrophilla is a major bacterial pathogen of intensive fresh water fish culture in Indonesia. An alternative method to control the pathogen is using probiotics. Probiotics is usually consist of live microorganisms which when administered in adequate amounts confer a health benefits on host. The aim of this research was to determine the probiotic candidates against A. hydrophilla which identified based on the 16S rDNA gene sequences. This research was started with field survey to obtained the probiotic candidate and continue with laboratory experiment. Probiotic candidates were isolated from fish pond water located in Boyolali, and Banjarnegara Regency, Central Java, Indonesia. A total of 133 isolates bacteria were isolated and cultured on to TSA, TSB and GSP medium. Out of 133 isolates only 30 isolates showed inhibition to A.hydrophilla activity. Three promising isolates were identified with PCR using primer for 16S rDNA. Based on 16S rDNA sequence analysis, all three isolates were belong to Bacillus genus. Isolate CKlA21, CKlA28, and CBA14 respectively were closely related to Bacillus sp. 13843 (GenBank accession no. JN874760.1-100% homology), Bacillus subtilis strain H13 (GenBank accession no.KT907045.1-99% homology), and Bacillus sp. strain 22-4 (GenBank accession no. KX816417.1-97% homology).