Biodegradation of diethyl phthalate by Pseudomonas sp. BZD-33 isolated from active sludge

2019 5th International Conference on Energy Materials and Environment Engineering

能源学;生态环境科学

Ke, Zhang^1 ; Xiangling, Wu^1 ; Jian, Chen^1 ; Jia, Chen^1

College of Civil Engineering, Sichuan Agricultural University, Dujiangyan, Sichuan

611830, China^1

关键词: 16S rDNA sequence;    Acidic conditions;    Alkaline conditions;    Degradation efficiency;    Degradation rate;    Degrading bacteria;    Diethyl phthalate;    Repair pathways;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/295/2/012070/pdf
DOI  :  10.1088/1755-1315/295/2/012070

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Diethyl phthalate (DEP) is a common pollutant, which is widely used in industry. A strain of DEHP-degrading bacteria was isolated from activated sludge.According to the phylogeny of 16S rDNA sequence, BDZ-33 strain was identified as pseudomonas sp. Biodegradation of DEP by Pseudomonas sp. BZD-33 was investigated. The results show that the BZD - 33 degradation DEP optimal pH and temperature of 8.0 and 15 °C respectively. The degradation concentration ranged from 100-400 mg•L-1. The degradation efficiency and growth rate of the strain were higher under alkaline conditions than under acidic conditions. The best temperature below 20 °C. The degradation rate decreases with the increase of temperature. After 60 h of culture, the degradation rate of DEP wastewater with a concentration of 400 mg/L reached 90%. The results showed that BDZ-33 could efficiently degrade DEP, which might be a promising DEP repair pathway.