Biodegradation of Diphenyl ortho-phthalate DPP by Pseudomonas sp. BET-7 isolated from active sludge

2018 International Conference of Green Buildings and Environmental Management

土木建筑工程;生态环境科学

Zhang, Ke^1 ; Chen, Jian^1 ; Yang, Siqiao^1 ; Chen, Wei^1 ; Chen, Zia^1

College of Civil Engineering, Sichuan Agricultural University, Dujiangyan, Sichuan

611830, China^1

关键词: 16S rDNA sequence;    Activated sludge;    Active sludges;    Bacterial strains;    Degradation efficiency;    Degradation rate;    Optimum temperature;    Sole source of carbon;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/186/3/012062/pdf
DOI  :  10.1088/1755-1315/186/3/012062

来源: IOP

【 摘 要 】

Diphenyl ortho-phthalate (DPP), as a prior pollutant for control, has been widely detected in environment. One bacterial strain was isolated from activated sludge using Diphenyl ortho-phthalate as the sole source of carbon and energy. According to the phylogeny of 16S rDNA sequence, the bacterial strain BET-7 was identified as Pseudomonas sp. Biodegradation of DPP by Pseudomonas sp. BET-7 was investigated. Results showed that the optimum pH and temperature for DPP degradation by BET-7 were 8.0 and 15 °C.The concentration of degradation ranged from 100 mg.L-1-400 mg.L-1. The strain exhibited higher degradation efficiencies and growth rates in alkalinity than in acidity. The optimum temperature was lower than 20 °C. The degradation rate decreased when temperature went up. The strain could degrade approximately 90% of 400 mg/L DPP after 60 h of cultivation. The results suggest that BET-7 may represent a promising application for DPP bioremediation.