Phylogenetic analyses of rhizobia isolated from Aeschynomene spp.

Biodiversitas

关键词: 16S rDNA sequence;    Aeshynomene sp.;    photosynthetic rhizobia;    DNA-DNA hybridization;    Rhodopseudomonas palustris;   

DOI  :  

来源: DOAJ

【 摘 要 】

The 16S rDNA sequence of eleven strains isolated from nodules of Aeschynomene spp. have been determined and analyses together with sequence of other related taxa. Four bacterial groups were identified: (i) photosynthetic Bradyrhizobial group consist of IRBG2, IRBG228 dan IRBG 230; (ii) non-photosintetik Bradyrhizobium group consist of MAFF210127, MAFF210316, MAFF210318, dan MAFF210408; (iii) Rhodopseudomonas palustris group consist of HMD88, HMD89 dan 99D and (4) an isolate, 99C, that is related to Rhodopseudomonas acidophila. In addition, DNA-DNA hybridization was performed among HMD88, HMD89 and 99D strains. The result showed that DNA homology of HMD88, HMD89 and 99D with Rps. palustris were less than 30%, DNA homology of HMD88, HMD89 and Rps. Palustris with 99D is about or less than 20%, otherwise between HMD88 and HMD89, DNA similarity was more than 70%. The result suggest that HMD88 and HMD89 are the same species, meanwhile 99D is close related or different species in Rhodopseudomonas palustris group.

【 授权许可】

Unknown