Identification of Bacterial Species in Kuwaiti Waters Through DNA Sequencing

2016 International Conference on Environmental Engineering and Sustainable Development

生态环境科学;经济学

Chen, K.^1

American International School of Kuwait, Hawalli, Kuwait^1

关键词: 16S rDNA sequence;    Amplification products;    Bacterial diversity;    Bacterial isolates;    Bacterial species;    Database projects;    Fecal coliforms;    Molecular identification;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/51/1/012015/pdf
DOI  :  10.1088/1742-6596/51/1/012015

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

With an objective of identifying the bacterial diversity associated with ecosystem of various Kuwaiti Seas, bacteria were cultured and isolated from 3 water samples. Due to the difficulties for cultured and isolated fecal coliforms on the selective agar plates, bacterial isolates from marine agar plates were selected for molecular identification. 16S rRNA genes were successfully amplified from the genome of the selected isolates using Universal Eubacterial 16S rRNA primers. The resulted amplification products were subjected to automated DNA sequencing. Partial 16S rDNA sequences obtained were compared directly with sequences in the NCBI database using BLAST as well as with the sequences available with Ribosomal Database Project (RDP).