The Effectiveness of Heterotrophic Bacteria Isolated from Dumai Marine Waters of Riau, Used as Antibacterial against Pathogens in Fish Culture

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Feliatra, F.^1 ; Nursyirwani^1 ; Tanjung, A.^2 ; Adithiya, D.S.^1 ; Susanna, M.^1 ; Lukystyowati, I.^3

Laboratory of Marine Microbiology, Faculty of Fisheries and Marine Sciences, University of Riau, Pekanbaru Riau Province

28286, Indonesia^1

Laboratory of Marine Biology, Faculty of Fisheries and Marine Sciences, University of Riau, Pekanbaru Riau Province

28286, Indonesia^2

Laboratory of Parasite and Diseases of Fish, Faculty of Fisheries and Marine Sciences, University of Riau, Pekanbaru Riau Province

28286, Indonesia^3

关键词: 16S rDNA sequence;    Antagonism;    Heterotrophic bacterias;    Marine waters;    Pathogenic bacterium;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012034/pdf
DOI  :  10.1088/1755-1315/116/1/012034

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Heterotrophic bacteria have an important role as decomposer of organic compounds (mineralization) derived from industrial waste, decomposition of unconsumed feed, faecal, excretion of fish, and have the ability to inhibit the growth of pathogenic bacteria. We investigated the role of heterotrophic bacteria used as antibacterial against pathogens in fish culture.This research was conducted from January until March 2017. The phylogenitic of the isolated bacterial was determined by 16S rDNA sequences analysis. Antagonism test showed that the bacteria had the ability to inhibit the growth of pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp.) Three isolates (Dm5, Dm6 and Dm4) indicated high inhibition zones which were classified into strong category with the average from 10.5 to 11.8 mm toward V. alginolitycus. Other isolates were classified into medium and weak category. Based on DNA analysis of heterotrophic bacteria isolated from marine waters of industrial area and low salinity of estuarine waters twelve strains of bacteria were identified, and all had highest level of homology to Bacillus sp.,one isolates has similarity to Enterobacter cloacae, other isolates to Clostridium cetobutylicum. Most of isolated bacteria obtained from the waters of industrial area due to it received much of nutrients that very influenced the growth of bacteria.