Fibrin Sealant Derived from Human Plasma as a Scaffold for Bone Grafts Associated with Photobiomodulation Therapy

International Journal of Molecular Sciences

RogérioLeone Buchaim¹  BrunaBotteon Della Coletta¹  AnaCarolina Cestari Biguetti¹  JoãoVitor Tadashi Cosin Shindo¹  AndréLuis Shinohara¹  DanielaVieira Buchaim¹  JesusCarlos Andreo¹  MarceliePriscila de Oliveira Rosso¹  KarinaTorres Pomini¹  MarcoAntônio Hungaro Duarte²  ÍrisJasmin Santos German³  MuriloPriori Alcalde⁴  GeraldoMarco Rosa Júnior⁴  Daniel de Bortoli Teixeira⁵ 

[1] Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of São Paulo (USP), Bauru 17012-901, Brazil;Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo (USP), Bauru 17012-901, Brazil;Department of Dentistry, Faculty of Health Science, Universidad Iberoamericana (UNIBE), Santo Domingo 10203, Dominic Republic;Department of Health Science, University of the Sacred Heart (USC), Bauru 17011-160, Brazil;Department of Human Morphophysiology, Medical and Dentistry School, University of Marilia (UNIMAR), Marília 17525-902, Brazil;

关键词: bone regeneration;    bone repair;    fibrin sealant;    biomaterial;    photobiomodulation therapy;    low-level laser therapy;   

DOI  :  10.3390/ijms20071761

来源: DOAJ

【 摘 要 】

Fibrin sealants derived from human blood can be used in tissue engineering to assist in the repair of bone defects. The objective of this study was to evaluate the support system formed by a xenograft fibrin sealant associated with photobiomodulation therapy of critical defects in rat calvaria. Thirty-six rats were divided into four groups: BC (n = 8), defect filled with blood clot; FSB (n = 10), filled with fibrin sealant and xenograft; BC^PBMT (n = 8), blood clot and photobiomodulation; FSB^PBMT (n = 10), fibrin sealant, xenograft, and photobiomodulation. The animals were killed after 14 and 42 days. In the histological and microtomographic analysis, new bone formation was observed in all groups, limited to the defect margins, and without complete wound closure. In the FSB group, bone formation increased between periods (4.3 ± 0.46 to 6.01 ± 0.32), yet with lower volume density when compared to the FSB^PBMT (5.6 ± 0.45 to 10.64 ± 0.97) group. It was concluded that the support system formed by the xenograft fibrin sealant associated with the photobiomodulation therapy protocol had a positive effect on the bone repair process.

【 授权许可】

Unknown