Biological Behavior of Xenogenic Scaffolds in Alcohol-Induced Rats: Histomorphometric and Picrosirius Red Staining Analysis

Polymers

Geraldo Marco Rosa Júnior¹  Beatriz Flavia de Moraes Trazzi²  Miguel Ângelo de Marchi³  Cláudio Maldonado Pastori⁴  André Luiz de Faria Figadoli⁵  Rogerio Leone Buchaim⁵  Iris Jasmin Santos German⁵  Marcelie Priscila de Oliveira Rosso⁵  Karina Torres Pomini⁵  Carlos Henrique Bertoni Reis⁵  Samira Salmeron⁶  Dayane Maria Braz Nogueira⁶  Mariana Schutzer Ragghianti Zangrando⁶  Patrícia Lopes Alcantara⁷  Paulo Sérgio da Silva Santos⁷  Jéssica de Oliveira Rossi⁸  Eliana de Souza Bastos Mazuqueli Pereira⁹  Daniela Vieira Buchaim⁹ 

[1] Anatomy Discipline, School of Dentistry, Health Sciences Center, Sacred Heart University Center (UNISAGRADO), Bauru 17011-160, Brazil;Coordination of the Dentistry School, Dentistry School, University of Marilia (UNIMAR), Marília 17525-902, Brazil;Coordination of the Medical School, Medical School, University Center of Adamantina (UniFAI), Adamantina 17800-000, Brazil;Dentistry School, University Center of Adamantina (UniFAI), Adamantina 17800-000, Brazil;Department of Biological Sciences, Bauru School of Dentistry (FOB/USP), University of São Paulo, Bauru 17012-901, Brazil;Department of Prosthodontics and Periodontics, Bauru School of Dentistry (FOB/USP), University of São Paulo, Bauru 17012-901, Brazil;Department of Surgery, Stomatology, Pathology and Radiology, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, Brazil;Graduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), São Paulo 05508-270, Brazil;Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marília 17525-902, Brazil;

关键词: bone regeneration;    alcohol intake;    scaffolds;    bone repair;    tibia;    xenograft;   

DOI  :  10.3390/polym14030584

来源: DOAJ

【 摘 要 】

In this experimental protocol, the objective was to evaluate the biological behavior of two xenogenic scaffolds in alcohol-induced rats through histomorphometric and Picrosirius Red staining analysis of non-critical defects in the tibia of rats submitted or not to alcohol ingestion at 25% v/v. Eighty male rats were randomly divided into four groups (n = 20 each): CG/B (water diet + Bio-Oss^® graft, Geistlich Pharma AG, Wolhusen, Switzerland), CG/O (water diet + OrthoGen^® graft, Baumer, Mogi Mirim, Brazil), AG/B (25% v/v alcohol diet + Bio-Oss^® graft), and AG/O (25% v/v alcohol diet + OrthoGen^® graft). After 90 days of liquid diet, the rats were surgically obtained, with a defect in the tibia proximal epiphysis; filled in according to their respective groups; and euthanized at 10, 20, 40 and 60 days. In two initial periods (10 and 20 days), all groups presented biomaterial particles surrounded by disorganized collagen fibrils. Alcoholic animals (AG/B and AG/O) presented, in the cortical and medullary regions, a reactive tissue with inflammatory infiltrate. In 60 days, in the superficial area of the surgical cavities, particles of biomaterials were observed in all groups, with new compact bone tissue around them, without complete closure of the lesion, except in non-alcoholic animals treated with Bio-Oss^® xenograft (CG/B), where the new cortical interconnected the edges of the defect. Birefringence transition was observed in the histochemical analysis of collagen fibers by Picrosirius Red, in which all groups in periods of 10 and 20 days showed red-orange birefringence, and from 40 days onwards greenish-yellow birefringence, which demonstrates the characteristic transition from the formation of thin and disorganized collagen fibers initially to more organized and thicker later. In histomorphometric analysis, at 60 days, CG/B had the highest volume density of new bone (32.9 ± 1.15) and AG/O the lowest volume density of new bone (15.32 ± 1.71). It can be concluded that the bone neoformation occurred in the defects that received the two biomaterials, in all periods, but the Bio-Oss^® was superior in the results, with its groups CG/B and AG/B displaying greater bone formation (32.9 ± 1.15 and 22.74 ± 1.15, respectively) compared to the OrthoGen^® CG/O and AG/O groups (20.66 ± 2.12 and 15.32 ± 1.71, respectively), and that the alcoholic diet interfered negatively in the repair process and in the percentage of new bone formed.

【 授权许可】

Unknown