The effects of recombinant vitronectin fragments on the human osteosarcoma cell line behavior

vitronectin;HOS cells;cell behavior;integrin;bone regeneration;617

치과대학 치의과학과 ;

University:서울대학교 대학원

关键词: vitronectin;    HOS cells;    cell behavior;    integrin;    bone regeneration;    617;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/131103/1/000000002902.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

amino acids 304-459) protein. Moreover, function-blocking inhibition assay using monoclonal antibody against integrin subunits revealed that αV integrin subunit played key a role in HOS cell adhesion, and αVβ3 and αVβ5 integrins acted as receptor of rVN-FI protein. rVN-FI-derived synthetic peptides containing Arg-Gly-Asp (RGD) motif, P6 (AECKPQVTRGDV) and P7 (PQVTRGDVFTMP) completely blocked the adhesion of HOS cell to VN. These results indicate that rVN-FI protein showed biological functions on osteoblast-like HOS cells. Thus, rVN-FI protein of human vitronectin may have potential application on bone tissue regeneration-related tissue engineering.