Materials Today Bio | |
Chorion-derived extracellular matrix hydrogel and fibronectin surface coatings show similar beneficial effects on endothelialization of expanded polytetrafluorethylene vascular grafts | |
Gabriela Eder1  Barbara Kapeller1  Marjan Enayati2  Karl H. Schneider2  Ursula Windberger2  Helga Bergmeister2  Sabrina Rohringer3  Pia Hager3  Bruno K. Podesser3  Herbert Kiss3  | |
[1] Austrian Cluster for Tissue Regeneration, Vienna, Austria;Ludwig Boltzmann Institute for Cardiovascular Research, Währinger Gürtel 18-20, 1090, Vienna, Austria;Medical University of Vienna, Center for Biomedical Research, Währinger Gürtel 18-20, 1090, Vienna, Austria; | |
关键词: Small diameter vascular grafts; ePTFE; Endothelialization; Human placental chorionic hydrogel; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
The endothelium plays an important regulatory role for cardiovascular homeostasis. Rapid endothelialization of small diameter vascular grafts (SDVGs) is crucial to ensure long-term patency. Here, we assessed a human placental chorionic extracellular matrix hydrogel (hpcECM-gel) as coating material and compared it to human fibronectin in-vitro. hpcECM-gels were produced from placental chorion by decellularization and enzymatic digestion. Human umbilical vein endothelial cells (HUVECs) were seeded to non-, fibronectin- or hpcECM-gel-coated expanded polytetrafluorethylene (ePTFE) SDVGs. Coating efficiency as well as endothelial cell proliferation, migration and adhesion studies on grafts were performed. hpcECM-gel depicted high collagen and glycosaminoglycan content and neglectable DNA amounts. Laminin and fibronectin were both retained in the hpcECM-gel after the decellularization process. HUVEC as well as endothelial progenitor cell attachment were both significantly enhanced on hpcECM-gel coated grafts. HUVECs seeded to hpcECM-gel depicted significantly higher platelet endothelial cell adhesion molecule-1 (PECAM-1) expression in the perinuclear region. Cell retention to flow was enhanced on fibronectin and hpcECM-gel coated grafts. Since hpcECM-gel induced a significantly higher endothelial cell adhesion to ePTFE than fibronectin, it represents a possible alternative for SDVG modification to improve endothelialization.
【 授权许可】
Unknown