Optimization Studies for Enhancing Cellulase Production by Penicillium janthinellum Mutant EU2D-21 Using Response Surface Methodology

BioResources

V. Ravi Kumar¹  Anil Kumar Nagraj²  Mamata Singhvi²  Digambar Gokhale² 

[1] Chemical Engineering and Process Development Division, CSIR-National Chemical Laboratory;NCIM Resource Center, National Chemical laboratory;

关键词: P. janthinellum EU2D-21;    Cellulase production;    Central composite design (CCD);    Plackett-Burman design (PBD);   

DOI  :  10.15376/biores.9.2.1914-1923

来源: DOAJ

【 摘 要 】

Extracellular fungal cellulases are key enzymes for the degradation of lignocellulosic biomass. Greater production of these enzymes could reduce the cost of biofuels production. In this study, the basal medium for cellulase production by a Penicillium janthinellum mutant (EU2D-21) in submerged fermentation conditions was optimized using response surface methodology (RSM). Initial studies using a Plackett-Burman design (PBD) showed that (NH4)2SO4 and urea are significant factors for improving β-glucosidase and FPase production. A central composite design (CCD) was applied to obtain the maximum response, which resulted in the optimal production of β-glucosidase (5.79 IU/mL) and FPase (5.76 IU/mL). These values were 1.87 and 1.67 times higher than the corresponding values obtained under un-optimized conditions.

【 授权许可】

Unknown