Characterization of thermostable cellulase produced by Bacillus strains isolated from solid waste of carrageenan

International Conference on Green Agro-industry and Bioeconomy 2017

农业科学;生物科学;经济学

Listyaningrum, N.P.^1,2 ; Sutrisno, A.^1 ; Wardani, A.K.^1

Department of Biotechnology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang, Indonesia^1

Fisheries Product Processing Technique, Marine and Fisheries Polytechnic, Sidoarjo, Indonesia^2

关键词: 16s rrna sequencing;    Bacillus licheniformis;    Carboxymethyl cellulose;    Cellulase production;    Lignocellulosic biomass;    Substrate specificity;    Thermostable cellulase;    Thermostable enzymes;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/131/1/012043/pdf
DOI  :  10.1088/1755-1315/131/1/012043

来源: IOP

【 摘 要 】

Cellulase-producing bacteria was isolated from solid waste of carrageenan and identified as Bacillus licheniformis C55 by 16S rRNA sequencing. The optimum condition for cellulase production was obtained at pH and temperature of 8.0 and 50°C, respectively in a medium containing glucose as carbon source and 1.0% carboxymethyl cellulose (CMC) to stimulate the cellulase production. Most remarkably, the enzyme retained its relative activity over 50% after incubation at 50°C for 90 minutes. Substrate specificity suggested that the enzyme is an endoglucanase. The molecular mass of Bacillus licheniformis C55 crude cellulase was found about 18 kDa by SDS-PAGE analysis. This thermostable enzyme would facilitate development of more efficient and cost-effective forms of the process to convert lignocellulosic biomass into high-value products.