Tracing the evolution of degraders in activated sludge during the sludge's acclimation to a xenobiotic organic

International Conference on Energy Engineering and Environmental Protection 2016

能源学;生态环境科学

Chong, N.M.^1 ; Fan, C.H.^1 ; Yang, Y.C.^1

Department of Environmental Engineering, DaYeh University, No. 168, University Road, Dacun, Changhua

51591, Taiwan^1

关键词: 16s rrna sequencing;    Activated sludge;    Community structures;    Continuous flow reaction;    Degradative pathways;    High throughput;    Sole carbon source;    Xenobiotic organic compounds;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/52/1/012107/pdf
DOI  :  10.1088/1742-6596/52/1/012107

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The molecular biology method of high-throughput pyrosequencing was employed to examine the change of activated sludge community structures during the process in which activated sludge was acclimated to and degraded a target xenobiotic. The sample xenobiotic organic compound used as the activated sludge acclimation target was the herbicide 2,4-dichlorphenoxyacetic acid (2,4-D). Indigenous activated sludge microorganisms were acclimated to 2,4-D as the sole carbon source in both the batch and the continuous-flow reaction modes. Sludge masses at multiple time points during the course of acclimation were subjected to pyrosequencing targeting the microorganisms' 16S rRNA genes. With the bacterial 16S rRNA sequencing results the genera that increased in abundance were checked with degradative pathway databases or literature to confirm that they are commonly seen as potent degraders of 2,4-D. From this systematic examination of degrader changes at time points during activated sludge acclimation and degradation of the target xenobiotic, the trend of degrader evolution in activated sludge over the sludge's acclimation process to a xenobiotic was traced.