Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives

Journal of Clinical Medicine

Martha Zakrzewski¹  David G. Armstrong²  Fatemah Sadeghpour Heravi³  Honghua Hu³  Karen Vickery³ 

[1] QIMR Berghofer Medical Research Institute, Brisbane QLD 4006, Australia;Southwestern Academic Limb Salvage Alliance (SALSA), Keck School of Medicine of University of Southern California (USC), Los Angeles, CA 90007, USA;Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney 2109, Australia;

关键词: diabetic foot ulcers;    diabetic foot infections;    microbiology;    culture;    culturomics;    16s rrna sequencing;    microbiota;    metagenomics;    metatranscriptomics;   

DOI  :  10.3390/jcm8111935

来源: DOAJ

【 摘 要 】

Diabetic foot ulcers (DFUs) and diabetic foot infections (DFIs) are associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. Diverse bacterial communities have been identified in DFUs/DFIs, playing a significant role in infection prognosis. However, due to the high heterogeneity of bacterial communities colonized in DFUs/DFIs, culture-based methods may not isolate all of the bacterial population or unexpected microorganisms. Recently, high sensitivity and specificity of DNA (metagenomics) and RNA (metatranscriptomics) technologies have addressed limitations of culture-based methods and have taken a step beyond bacterial identification. As a consequence, new advances obtained from DNA- and RNA-based techniques for bacterial identification can improve therapeutic approaches. This review evaluated the current state of play in aetiology of DFUs/DFIs on culture and molecular approaches, and discussed the impact of metagenomic and metatranscriptomic methods in bacterial identification approaches.

【 授权许可】

Unknown