Preliminary plant design of Escherichia coli BPPTCC-EgRK2 cell culture for recombinant cellulase production using Oil Palm Empty Fruit Bunch (OPEFB) as substrate

2nd International Conference on Biomass: Toward Sustainable Biomass Utilization for Industrial and Energy Applications

生物科学;工业技术;能源学

Surya, E.A.^1 ; Rahman, S.F.^1 ; Zulamraini, S.^1 ; Gozan, M.^1

Chemical Engineering Department, Faculty of Engineering, Universitas Indonesia, Kampus UI, Depok

16424, Indonesia^1

关键词: Alkaline pretreatment;    Cellulase production;    Fermentation inhibitors;    Mass and energy balance;    Oil palm empty fruit bunch (OPEFB);    Pretreatment methods;    Pretreatment process;    Technology evaluation;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/141/1/012030/pdf
DOI  :  10.1088/1755-1315/141/1/012030

来源: IOP

【 摘 要 】

An economic analysis of recombinant cellulase production from E. coli BPPTCC Eg-RK2 was conducted to support the fulfilling of Indonesia's energy roadmap for ethanol production. The plant use oil palm empty fruit bunch (OPEFB) as primary substrate in cellulase production, with the expected lifetime of 12 years. The plant is assumed to be built in Indonesia and will fulfill 1% of total market demand. The effect of different pretreatment process (alkaline, steam explosion, and sequential acid-alkaline) on the economic value was also studied. A simulation using SuperPro Designer was used to calculate the mass and energy balance based on the kinetic parameter of E. coli BPPTCC-EgRK2. Technology evaluation show that alkaline pretreatment gave the highest yield with no known inhibitors formed. The steam explosion show the lowest lignin and hemicellulose removal and known to form known fermentation inhibitors. The net present value of alkaline, steam explosion, and sequential acid-alkaline pretreatment were USD 7,118,000; - USD 73,411,000 and USD -114,013,000 respectively, which mean alkaline pretreatment is the only economically feasible pretreatment method for recombinant cellulase production.