Enhancement of Cellulase (CMCase) production from marine actinomycetes Streptomyces sp. Bse 7-9: Optimization of fermentation medium by Response Surface Methodology

2nd International Conference on Natural Products and Bioresource Sciences - 2018

生物科学;生态环境科学

Ratnakomala, S.^1 ; Fahrurrozi^1 ; Yopi^1

Research Center for Biotechnology, Indonesian Institute of Sciences, Jl Raya Bogor km 46, Cibinong

16911, Indonesia^1

关键词: Carboxymethyl cellulase;    Cellulase production;    Central composite designs;    Fermentation conditions;    Fermentation medium;    Optimal concentration;    Response surface methodology;    Taguchi experimental design;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/251/1/012005/pdf
DOI  :  10.1088/1755-1315/251/1/012005

来源: IOP

【 摘 要 】

Optimization of fermentation conditions is important in the development of bioprocesses. Here, we used the Taguchi experimental design and Response Surface Methodology to optimize fermentation medium for enhancing carboxymethyl cellulase (CMCase) production by marine actinomycetes Streptomyces sp. Bse 7-9. Optimization using the Taguchi experimental design we found that bagasse has a significant effect on other factors. The Central Composite Design was used to determine the interaction between the factors selected in the fermentation medium to obtain the optimal concentration of ingredients. The experimental results demonstrated that bagasse as a carbon source plays as the key component for the production of CMCase by marine actinomycetes Streptomyces sp. Bse 7-9. In conclusion, the optimal fermentation medium for maximum cellulase production by marine actinomycete Streptomyces sp. Bse 7-9 was as follows: bagasse concentration (1.84 % w/v), yeast extract (0.5 % w/v) and CaSO4 (0.02 % w/v). The maximum activity of CMCase at this optimum medium was 4.496 U mL-1.