Kinetic study of Escherichia coli BPPTCC-EgRK2 to produce recombinant cellulase for ethanol production from oil palm empty fruit bunch

2nd International Conference on Biomass: Toward Sustainable Biomass Utilization for Industrial and Energy Applications

生物科学;工业技术;能源学

Limoes, S.^1 ; Rahman, S.F.^1 ; Setyahadi, S.^2 ; Gozan, M.^1

Chemical Engineering Department, Faculty of Engineering, University of Indonesia, Depok

16424, Indonesia^1

Center for Bioindustrial Technology, Agency for Assessment and Application of Technology, Serpong

15314, Indonesia^2

关键词: Alternative substrates;    Bacteria cultivations;    Cell concentrations;    Cellulase production;    Ethanol production;    Maximum specific growth rates;    Oil palm empty fruit bunch;    Oil palm empty fruit bunch (OPEFB);   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/141/1/012016/pdf
DOI  :  10.1088/1755-1315/141/1/012016

来源: IOP

【 摘 要 】

Oil Palm Empty Fruit Bunch (OPEFB) is an abundant biomass resource in Indonesia, which contains 46,77% (w/w) of cellulose. The high cellulose content of OPEFB can be used as a substrate for bacteria cultivation to produce cellulase. By using OPEFB as an alternative substrate, the production cost of cellulase in industrial scale can be suppressed. However, currently there are no available research that simulate a cellulase production plant design. Prior to simulating the cellulase plant design, kinetic studies of bacteria used in cultivation are needed to create an accurate simulation. In this research, kinetic studies of E. coli BPPTCC-EgRK2 growth were examined with the Monod approach to get the Monod constant (Ks) and maximum specific growth rate (μmax). This study found that E. coli BPPTCC-EgRK2 have μmax and Ks of 1.581 and 0.0709 respectively. BPPTCC-EgRK2 produced intracellular cellulase, thus gave linear correlation between cell concentration and cellulase production.