Phycocyanin extraction in Spirulina produced using agricultural waste

29th Symposium of Malaysian Chemical Engineers 2016

Taufiqurrahmi, N.^1 ; Religia, P.^2 ; Mulyani, G.^1 ; Suryana, D.^4 ; Ichsan^4 ; Tanjung, F.A.^5 ; Arifin, Y.^3,5

Chemical Engineering Department, Surya University, Boulevard Gading Serpong, Tangerang

15810, Indonesia^1

Department of Food Technology, International University Liaison Indonesia, IULI, Eco Campus, Breeze BSD City, Tangerang Selatan

15345, Indonesia^2

Department of Food Business Technology, Prasetiya Mulya University, BSD Raya Utama, BSD City, Kabupaten Tangerang

15339, Indonesia^3

PT Maris Sustainable Indonesia, Jl Tipar Cakung, Pool PPD 5, Cakung, Jakarta Timur

13910, Indonesia^4

Department of Chemical Engineering, Curtin University Sarawak Campus, CDT 250, Sarawak, Miri

98009, Malaysia^5

关键词: Alternative substrates;    Arthrospira platensis;    Cell concentrations;    Food industries;    Pigment-protein complexes;    Potential health;    Synthetic chemicals;    Triple superphosphates;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/206/1/012097/pdf
DOI  :  10.1088/1757-899X/206/1/012097

来源: IOP

【 摘 要 】

Phycocyanin is a pigment-protein complex synthesized by blue-green microalgae such as Arthrospira (Spirulina) platensis. This pigment is used mainly as natural colouring in food industry. Previous studies have demonstrated the potential health benefits of this natural pigment. The price of phycocyanin is a vital factor that dictates its marketability. The cost of culturing the algae, particularly from the substrate used for growth, is one of the main factors that determine the price of phycocyanin. Another important factor is the growth yield of the algae. In our research, agricultural waste such as charcoal produced from rice husk was utilized for the algae cultivation to replace the synthetic chemicals such as urea and triple superphosphate used the mineral medium. The use of this low cost substrate increases the cell concentration by 60 % during 8 days' cultivation to reach 0.39 g/l. The phycocyanin extraction was performed using water at the different biomass-to-solvent ratio and shaking rates. The phycocyanin concentration and purity (A615/A280) obtained were 1.2 g/l and 0.3. These values are 40 % and 20 % lower than the value obtained from the algae produced using the synthetic chemicals. Further purification produced the extract purity required for food grade. The biomass-solvent ratio does not significantly affect the extract purity; however, the higher shaking rate during extraction reduces the purity. This finding demonstrates the potential of using rice husk as an alternative substrate to cultivate algae for phycocyanin extraction.