Alternative to FBS in animal cell culture - An overview and future perspective

Heliyon

Saravanakumar Venkatachalam¹  Jackson Durairaj Selvan Christyraj²  Niranjan Chellathurai Vasantha²  Beryl Vedha Yesudhason²  Manikandan Mohan³  Kamarajan Rajagopalan³  Johnson Retnaraj Samuel Selvan Christyraj³  Karthikeyan Subbiahanadar Chelladurai³ 

[1] Corresponding author.;Technology, Chennai, Tamilnadu, India;;Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science &

关键词: Fetal bovine serum;    Animal cell culture;    Human platelet lysate;    Coelomic fluid;    Earthworm;    Alternative to FBS;   

DOI  :  

来源: DOAJ

【 摘 要 】

Fetal bovine serum (FBS) is a widely used growth supplement in the in vitro culturing of animal and human cells, tissues and organs, notably due to the occurrence of abundant micro- and macronutrients, along with growth factors. Over the years, increasing demand, high price, batch-to-batch variability in quality and composition, increasing ethical concerns lead to the search for an alternative to FBS. Several approaches have been suggested and employed in the past, but none is implemented as widely as FBS, and each supplement has its own disadvantages. In this review, we described the importance of FBS in cell culture, discussed the issues associated with FBS use and presented the efforts made in the recent past to reduce or replace FBS. The potential of four different alternative sources to FBS, namely, bovine ocular fluid, sericin protein, human platelet lysate and earthworm heat inactivated coelomic fluid was evaluated. In the end, we present the conceptual perspective using the Human Platelet Lysate (HPL) and earthworm Heat Inactivated Coelomic Fluid (HI-CF) combination to alternate FBS and its context in scientific and economic impacts.

【 授权许可】

Unknown