An NMR‐based metabonomic approach to the investigation of coelomic fluid biochemistry in earthworms under toxic stress

FEBS Letters

Nicholson, J.K²  Bundy, J.G²  Osborn, D¹  Weeks, J.M¹  Lindon, J.C² 

[1] CEH Monks Wood, Abbots Ripton, Huntingdon PE28 2LS, UK;Biological Chemistry, Biomedical Sciences Division, Imperial College of Science, Medicine, and Technology, Sir Alexander Fleming Building, South Kensington, London SW7 2AZ, UK

关键词: Metabonomics;    3-Fluoro-4-nitrophenol;    Coelomic fluid;    Eisenia veneta;    TMAO;    trimethylamine-N-oxide;    NMN;    nicotinamide mononucleotide;    NMR;    nuclear magnetic resonance;    3F4NP;    3-fluoro-4-nitrophenol;   

DOI  :  10.1016/S0014-5793(01)02582-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The endogenous metabolites of the coelomic fluid of the earthworm Eisenia veneta were characterised using high-resolution one-dimensional and two-dimensional ¹H nuclear magnetic resonance spectroscopy. Signals from common organic acids, such as acetate, fumarate, malonate, malate, formate, and succinate, were identified together with adenosine and nicotinamide mononucleotide. The potential use of this information as a baseline dataset for future toxicological or physiological studies was demonstrated by a metabonomic analysis: a series of earthworms were dosed with the model compound 3-fluoro-4-nitrophenol, and toxic effects followed by multivariate analysis of the spectral data of the coelomic fluid. Relative concentrations of acetate and malonate were decreased in the dosed worms compared to the controls.

【 授权许可】

Unknown