High tetragonula sp honey addition reduce cell proliferation on fibroblast preputium culture

Tarumanagara International Conference on the Applications of Technology and Engineering

工业技术（总论）

Shafira, M.^1 ; Pramono, A.^2 ; Nugrohowati, N.^2 ; Sahlan, M.^3

Sarjana Kedokteran, Medicine Faculty, Universitas Pembangunan Nasional Veteran Jakarta, Jl. RS Fatmawati, Pondok Labu, Jakarta Selatan

12450, Indonesia^1

Medicine Faculty, Universitas Pembangunan Nasional Veteran Jakarta, Jl. RS Fatmawati, Pondok Labu, Jakarta Selatan

12450, Indonesia^2

Chemical Engineering Faculty, Universitas Indonesia, Depok, Jawa Barat, Indonesia^3

关键词: Culture medium;    Experimental methods;    Fetal bovine serum;    Fibroblast cells;    Induced pluripotent stem cells;    Protein transduction;    Skin tissue;    Tissue explants;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/508/1/012146/pdf
DOI  :  10.1088/1757-899X/508/1/012146

学科分类：工业工程学

来源: IOP

【 摘 要 】

Induced pluripotent stem cells can be engineered by protein transduction from fibroblast cell, that has been obtained from skin tissue. To increase the protein transduction, needs optimization culture medium. Fetal Bovine Serum was replaced by honey from Tetragonola sp.To reduce the protease and increased the protein transduction efficiency. The purposed of this preliminary research were to determine the comparison between DMEM media-free serum addition of Tetragonula sp honey on fibroblast preputium cell proliferation. The design of this study used true experimental method. The sample of preputium skin was taken from a healthy child