Human platelet lysate to substitute fetal bovine serum in hMSC expansion for translational applications: a systematic review

Journal of Translational Medicine

M. O. Riehle¹  A. M. Hart²  W. Raffoul³  P. G. di Summa³  M. Guiotto⁴ 

[1] Centre for the Cellular Microenvironment, University of Glasgow, Glasgow, UK;Centre for the Cellular Microenvironment, University of Glasgow, Glasgow, UK;Canniesburn Plastic Surgery Unit, Glasgow Royal Infirmary, Glasgow, UK;Department of Plastic, Reconstructive and Hand Surgery, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland;Department of Plastic, Reconstructive and Hand Surgery, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland;Centre for the Cellular Microenvironment, University of Glasgow, Glasgow, UK;

关键词: Human platelet lysate;    Foetal bovine serum;    Mesenchymal stem cells;    Adipose derived stem cells;    Cell therapy;   

DOI  :  10.1186/s12967-020-02489-4

来源: Springer

【 摘 要 】

BackgroundFoetal bovine serum (FBS), is the most commonly used culture medium additive for in vitro cultures, despite its undefined composition, its potential immunogenicity and possible prion/zoonotic transmission. For these reasons, significant efforts have been targeted at finding a substitute, such as serum free-media or human platelet-lysates (hPL). Our aim is to critically appraise the state-of-art for hPL in the published literature, comparing its impact with FBS.Materials and methodsIn June 2019 a systematic search of the entire Web of Science, Medline and PubMed database was performed with the following search terms: (mesenchymal stem cells) AND (fetal bovine serum OR fetal bovine calf) AND (human platelet lysate). Excluded from this search were review articles that were published before 2005, manuscripts in which mesenchymal stem cells (MSCs) were not from human sources, and when the FBS controls were missing.ResultsBased on our search algorithm, 56 papers were selected. A review of these papers indicated that hMSCs cultured with hPL showed a spindle-shaped elongated morphology, had higher proliferation indexes, similar cluster of differentiation (CD) markers and no significant variation in differentiation lineage (osteocyte, adipocyte, and chondrocyte) compared to those cultured with FBS. Main sources of primary hMSCs were either fat tissue or bone marrow; in a few studies cells isolated from alternative sources showed no relevant difference in their response.ConclusionDespite the difference in medium choice and a lack of standardization of hPL manufacturing, the majority of publications support that hPL was at least as effective as FBS in promoting adhesion, survival and proliferation of hMSCs. We conclude that hPL should be considered a viable alternative to FBS in hMSCs culture—especially with a view for their clinical use.

【 授权许可】

CC BY