Assessment of MALDI TOF MS as alternative tool for Streptococcus suis identification

Frontiers in Public Health

Teresa eGarcía-Seco¹  Lucas eDominguez¹  Ana Isabel Vela¹  JOSE FRANCISCO FERNANDEZ-GARAYZABAL¹  Marta ePerez-Sancho¹  Marcelo eGottschalk² 

[1] Facultad de Veterinaria, Universidad Complutense;Faculté de Médecine, Vétérinaire, Université de Montréal;

关键词: Streptococcus suis;    PCR;    IDENTIFICATION;    MALDI-TOF MS;    biochemical markers;   

DOI  :  10.3389/fpubh.2015.00202

来源: DOAJ

【 摘 要 】

The accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identifying Streptococcus suis isolates obtained from pigs, wild animals and humans was evaluated using a PCR-based identification assay as the gold standard. In addition, MALDI-TOF MS was compared with the commercial multi-tests Rapid ID 32 STREP system. From the 129 S. suis isolates included in the study and identified by the molecular method, only 31 isolates (24.03%) had score values ≥2.300 and 79 isolates (61.24%) gave score values between 2.299-2.000. After updating the currently available S. suis MALDI Biotyper database with the spectra of three additional clinical isolates of serotypes 2, 7 and 9, most isolates had statistically significant higher score values (mean score: 2.65) than those obtained using the original database (mean score: 2.182). Considering the results of the present study, we suggest using a less restrictive threshold score of ≥2.000 for reliable species identification of S. suis. According to this cut-off value, a total of 125 S. suis isolates (96.9%) were correctly identified using the updated database. These data indicate an excellent performance of MALDI-TOF MS for the identification of S. suis.

【 授权许可】

Unknown