Development of PCR for identifying Streptococcus parasuis, a close relative of Streptococcus suis

The Journal of Veterinary Medical Science

Sakura ARAI¹  Ryohei NOMOTO²  Mari TOHYA³  Le Hong Thuy TIEN⁴  Kasumi ISHIDA-KUROKI⁵  Ryoko YAMADA⁶ 

[1] Department of Biotechnology, Nong Lam University, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam;Department of Infectious Diseases, Kobe Institute of Health, Minatojima-Nakamachi 4-6-5, Chuo-ku, Kobe, Hyogo 650-0045, Japan;Present address: Division of Microbiology, National Institute of Health Sciences, Tonomachi 3-25-26, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan;Present address: Laboratory of Veterinary Ethology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan;Present address: Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, Toyama 1-21-1, Shinjuku-ku, Tokyo 162-8655, Japan;Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan

关键词: PCR;    pig saliva;    recN;    Streptococcus parasuis;    Streptococcus suis;   

DOI  :  10.1292/jvms.18-0083

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Streptococcus parasuis has recently been removed taxonomically from Streptococcus suis, a zoonotic pathogen. S. parasuis has been detected in healthy pigs and in diseased pigs, which suggests that S. parasuis is involved in the normal microbiota of pigs and has potential pathogenicity. However, the pathogenicity of S. parasuis in pigs is unclear because of the lack of appropriate detection methods that discriminate S. parasuis from S. suis. In this study, we developed a PCR method that is specific for S. parasuis. The detection limit of the PCR was 350 CFU per reaction. Bacteria isolated from the saliva of eight pigs were collected and examined by PCR. Sixty-four isolates positive for PCR were obtained from the samples of all pigs. Thirteen of the 64 isolates were genetically confirmed as S. parasuis, and biologically and biochemically had nearly the same features of known S. parasuis strains, which suggested that strains positive for PCR were S. parasuis. Among the 64 isolates, 28 isolates were serotypes 20, 22, or 26 in the S. suis serotyping scheme. The remaining 36 isolates were untypeable, which suggested the presence of novel serotypes or a capsule-negative form. Therefore, the PCR method described in this study is a useful tool for identifying S. parasuis, and can be used in etiological studies on this bacterium.

【 授权许可】

Unknown