MALDI-TOF MS Identification of Peptide Citrullination Site Using Br Signature

citrullination;deimination;citrulline;4-bromophenyl glyoxal;MALDI-TOF MS;540

자연과학대학 화학부 ;

University:서울대학교 대학원

关键词: citrullination;    deimination;    citrulline;    4-bromophenyl glyoxal;    MALDI-TOF MS;    540;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/134875/1/000000008206.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

Enzymatic conversion of arginine to citrulline by peptidyl arginine deiminase (PAD) is associated with peptide presentation and development of autoimmunity in rheumatoid arthritis. In order to facilitate identification of the citrullination site, citrulline residue was modified using 4-bromophenyl glyoxal (BPG), and 194 Da mass increase and incorporation of the Br signature were confirmed by MALDI-TOF MS. Using this approach, we identified four and five citrullination sites of bovine serum albumin (BSA) and bovine fibrinogen, respectively. MALDI-TOF/TOF MS was used to unambiguously identify two citrullination sites from bovine fibrinogen.