C/EBPβ converts bovine fibroblasts to adipocytes without hormone cocktail induction

Electronic Journal of Biotechnology

Sayed Haidar Abbas Raza¹  Gong Cheng²  Linsen Zan²  Feng Long²  Yuan Tian²  Rajwali Khan²  Abdullah F. Shater²  Hong Wang³  Zuhair M. Mohammedsaleh⁴  Li Wang⁵ 

[1] National Beef Cattle Improvement Centre, Yangling, 712100, Shaanxi, PR China;College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, PR China;Department of Livestock Management, Breeding and Genetics The University of Agriculture Peshawar, Pakistan;Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Saudi Arabia;Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences. University of Tabuk, Tabuk 71491, Saudi Arabia;

关键词: Adipogenesis;    Adipogenic genes;    C/EBPβ;    Cattle;    Fibroblasts;    Fibrogenesis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: Adipogenesis and fibrogenesis can be considered as a competitive process in muscle, which may affect the intramuscular fat deposition. The CCAAT/enhancer-binding protein beta (C/EBPβ) plays an important role in adipogenesis, which is well-characterized in mice, but little known in bovine so far. Results: In this study, real-time qPCR revealed that the level of C/EBPβ was increased during the developmental stages of bovine and adipogenesis process of preadipocytes. Overexpression of C/EBPβ promoted bovine fibroblast proliferation through mitotic clonal expansion (MCE), a necessary process for initiating adipogenesis, by significantly downregulating levels of p21 and p27 (p < 0.01). Also, the PPARγ expression was inhibited during the MCE stage (p < 0.01). 31.28% of transfected fibroblasts adopted lipid-laden adipocyte morphology after 8 d. Real-time qPCR showed that C/EBPβ activated the transcription of early stage adipogenesis markers C/EBPα and PPARγ. Expression of ACCα, FASN, FABP4 and LPL was also significantly upregulated, while the expression of LEPR was weakened. Conclusions: It was concluded C/EBPβ can convert bovine fibroblasts into adipocytes without hormone induction by initiating the MCE process and promoting adipogenic genes expression, which may provide new insights into the potential functions of C/EBPβ in regulating intramuscular fat deposition in beef cattle.How to cite: Cheng G, Raza SHA, Khan R, et al. C/EBPβ converts bovine fibroblasts to adipocytes without hormone cocktail induction. Electron J Biotechnol 2021;52. https://doi.org/10.1016/j.ejbt.2021.04.008

【 授权许可】

Unknown