Differential adrenergic regulation of C/EBPα and C/EBPβ in brown adipose tissue

FEBS Letters

Rehnmark, Stefan²  Jacobsson, Anders²  Antonson, Per¹  Xanthopoulos, Kleanthis G.¹ 

[1] Karolinska Institute, Center for Biotechnology, NOVUM, S-141 57 Huddinge, Sweden;The Wenner-Gren Institute, The Arrhenius Laboratories F3, Stockholm University, S-106 91 Stockholm, Sweden

关键词: Gene expression regulation;    Differentiation;    Transcription factor;    C/EBPα;    C/EBPβ;    Brown adipose tissue;    Mouse;   

DOI  :  10.1016/0014-5793(93)80519-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the regulation of the expression of two members of the C/EBP family of transcriptional activators, C/EBPα and C/EBPβ, in brown adipose tissue in mice. Less than one hour of cold exposure led to dramatic changes in the expression of both genes. C/EBPα steady-state mRNA and protein levels were drastically and rapidly reduced whereas C/EBPβ mRNA and protein levels were induced severalfold. Also norepinephrine injection affected the expression of the transcription factors. Preconfluent cells in brown fat primary cultures responded to norepinephrine with a decrease in C/EBPα and an increase in C/EBPβ mRNA; in confluent cells the expression of both factors was increased. Thus, C/EBPα and C/EBPβ gene expression is under adrenergic control both in vivo and in vitro but the type of response is directed by the degree of differentiation of the cells.

【 授权许可】

Unknown