A mouse renin promoter containing the conserved decanucleotide element binds the same B‐cell factors as an authentic immunoglobulin heavy chain promoter

FEBS Letters

Ekker, Marc¹  Doyen, Noelle¹  Leblond-Francillard, Marie¹  Rougeon, François¹ 

[1] Unité de Génétique et Biochimie du Développement, LA CNRS 361, Institut Pasteur, 25, rue du Dr. Roux, F-75724 Paris Cédex 15, France

关键词: Transcription factor;    Promoter region;    Immunoglobulin;    Gene expression regulation;    dc/cd;    decanucleotide element;    Ig;    immunoglobulin;    poly(dI-dC);    poly(deoxyinosine-deoxy-cytosine);    Ren;    renin;    VH promoter;    immunoglobulin heavy chain promoter;   

DOI  :  10.1016/0014-5793(87)80397-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A mouse renin-1 gene promoter fragment, normally inactive in B-cells, becomes a potent promoter in these cells after insertion of the highly conserved decanucleotide (dc/cd sequence) of immunoglobulin heavy and light chain promoters [(1987) EMBO J. 6, 1685–1690]. We observe retarded complexes of the same electrophoretic mobility when the cd-containing renin promoter fragment or an authentic immunoglobulin heavy chain promoter fragment is incubated with a nuclear extract from myeloma cells, suggesting that the renin promoter is activated due to its acquired ability to bind a B-cell-specific positive factor. No retarded complexes are observed with the original renin promoter fragment thus questioning the presence of a repressor as an explanation for its lack of activity in B-cells.

【 授权许可】

Unknown