FEBS Letters | |
Nuclear factor Y activates the human xanthine oxidoreductase gene promoter | |
Lapatto, Risto2  Martelin, Eeva2  Raivio, Kari O.2  Palvimo, Jorma J.1  | |
[1] Department of Physiology, Institute of Biomedicine, P.O. Box 9, University of Helsinki, 00014 Helsinki, Finland;Hospital for Children and Adolescents, Research Laboratory, University of Helsinki, P.O. Box 281, 00290 Helsinki, Finland | |
关键词: Xanthine oxidase; Xanthine dehydrogenase; Gene regulation; Promoter region; Transcription factor; Nuclear factor Y; CBF; CCAAT binding factor; CDP; CCAAT displacement protein; C/EBP; CCAAT/enhancer binding protein; EMSA; electrophoretic mobility shift assay; NF1; nuclear factor 1; NF-Y; nuclear factor Y; XOR; xanthine oxidoreductase; | |
DOI : 10.1016/S0014-5793(00)01909-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
To study the regulation of the human xanthine oxidoreductase (XOR) gene, we cloned 1.9 kb of the promoter region. In reporter gene assays, a construct encompassing nucleotides between −142 to +42 conferred maximal basal activity of the XOR promoter in 293T cells, in comparison with shorter (−92 to +42) or longer (up to −1937 to +42) constructs. The promoter activity was low in NIH-3T3 cells. The most active construct contained a putative CCAAT motif at −119 to −123. Electrophoretic mobility shift assays showed that this sequence binds the ubiquitous nuclear factor Y (NF-Y). Mutation of the CCAAT motif (CTGAT) abolished the NF-Y binding and considerably reduced the promoter activity. Our data suggest an important functional role for NF-Y in the transcriptional activation of the human XOR gene.
【 授权许可】
Unknown
【 预 览 】
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