Nuclear factor Y activates the human xanthine oxidoreductase gene promoter

FEBS Letters

Lapatto, Risto²  Martelin, Eeva²  Raivio, Kari O.²  Palvimo, Jorma J.¹ 

[1] Department of Physiology, Institute of Biomedicine, P.O. Box 9, University of Helsinki, 00014 Helsinki, Finland;Hospital for Children and Adolescents, Research Laboratory, University of Helsinki, P.O. Box 281, 00290 Helsinki, Finland

关键词: Xanthine oxidase;    Xanthine dehydrogenase;    Gene regulation;    Promoter region;    Transcription factor;    Nuclear factor Y;    CBF;    CCAAT binding factor;    CDP;    CCAAT displacement protein;    C/EBP;    CCAAT/enhancer binding protein;    EMSA;    electrophoretic mobility shift assay;    NF1;    nuclear factor 1;    NF-Y;    nuclear factor Y;    XOR;    xanthine oxidoreductase;   

DOI  :  10.1016/S0014-5793(00)01909-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To study the regulation of the human xanthine oxidoreductase (XOR) gene, we cloned 1.9 kb of the promoter region. In reporter gene assays, a construct encompassing nucleotides between −142 to +42 conferred maximal basal activity of the XOR promoter in 293T cells, in comparison with shorter (−92 to +42) or longer (up to −1937 to +42) constructs. The promoter activity was low in NIH-3T3 cells. The most active construct contained a putative CCAAT motif at −119 to −123. Electrophoretic mobility shift assays showed that this sequence binds the ubiquitous nuclear factor Y (NF-Y). Mutation of the CCAAT motif (CTGAT) abolished the NF-Y binding and considerably reduced the promoter activity. Our data suggest an important functional role for NF-Y in the transcriptional activation of the human XOR gene.

【 授权许可】

Unknown