A new route to peroxynitrite: a role for xanthine oxidoreductase

FEBS Letters

Harrison, Roger¹  Durgan, Joanne¹  Godber, Ben L.J¹  Doel, Justin J¹  Eisenthal, Robert¹ 

[1] Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK

关键词: Xanthine oxidase;    Peroxynitrite;    Nitrite;    Superoxide;    Nitric oxide;    Xanthine dehydrogenase;    XOR;    xanthine oxidoreductase;    XO;    xanthine oxidase;    XDH;    xanthine dehydrogenase;    NO;    nitric oxide;    DHR;    dihydrorhodamine 123;    IDP;    iodonium diphenyl;    SOD;    superoxide dismutase;   

DOI  :  10.1016/S0014-5793(00)01639-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peroxynitrite, a potent oxidising, nitrating and hydroxylating agent, results from the reaction of nitric oxide with superoxide. We show that peroxynitrite can be produced by the action of a single enzyme, xanthine oxidoreductase (XOR), in the presence of inorganic nitrite, molecular oxygen and a reducing agent, such as pterin. The effects of oxygen concentration on peroxynitrite production have been examined. The physiologically predominant dehydrogenase form of the enzyme is more effective than the oxidase form under aerobic conditions. It is proposed that XOR-derived peroxynitrite fulfils a bactericidal role in milk and in the digestive tract.

【 授权许可】

Unknown