Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa

BMC Biotechnology

Khosro Zamani¹  Rezvan Golmoradi¹  Gholam-Reza Irajian¹  Shabnam Razavi¹  Noushin Allah-Bakhshi²  Faezeh Akhavan²  Mahdieh Yousefi²  Mahyar Gerami²  Moazzameh Ramezani³  Majid Tafrihi⁴  Horacio Bach⁵  Fatemeh Ramezani⁶  Ali Pakdin-Parizi⁷ 

[1] 1. Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran. 2. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;Department of Biology, Sana Institute of Higher education, Sari, Iran;Department of Biology, Urmia University, Urmia, Iran;Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran;Division of Infectious Diseases, Department of Medicine, University of British Columbia, Vancouver, BC, Canada;Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran;Sari Agricultural Sciences and Natural Resources University, Sari, Iran;

关键词: Cerium oxide nanoparticles;    Nanofiber;    Antibiotic resistance;    Pseudomonas aeruginosa;    Gene expression;    Cytotoxicity;    Clinical isolate;   

DOI  :  10.1186/s12896-021-00727-1

来源: Springer

【 摘 要 】

BackgroundAntibiotics have been widely used for the treatment of bacterial infections for decades. However, the rapid emergence of antibiotic-resistant bacteria has created many problems with a heavy burden for the medical community. Therefore, the use of nanoparticles as an alternative for antibacterial activity has been explored. In this context, metal nanoparticles have demonstrated broad-spectrum antimicrobial activity. This study investigated the antimicrobial activity of naked cerium oxide nanoparticles dispersed in aqueous solution (CNPs) and surface-stabilized using Pseudomonas aeruginosa as a bacterial model.MethodsGelatin-polycaprolactone nanofibers containing CNPs (Scaffold@CNPs) were synthesized, and their effect on P. aeruginosa was investigated. The minimum inhibitory and bactericidal concentrations of the nanoparticls were determined in an ATCC reference strain and a clinical isolate strain. To determine whether the exposure to the nanocomposites might change the expression of antibiotic resistance, the expression of the genes shv, kpc, and imp was also investigated. Moreover, the cytotoxicity of the CNPs was assessed on fibroblast using flow cytometry.ResultsMinimum bactericidal concentrations for the ATCC and the clinical isolate of 50 µg/mL and 200 µg/mL were measured, respectively, when the CNPs were used. In the case of the Scaffold@CNPs, the bactericidal effect was 50 µg/mL and 100 µg/mL for the ATCC and clinical isolate, respectively. Interestingly, the exposure to the Scaffold@CNPs significantly decreased the expression of the genes shv, kpc, and imp.ConclusionsA concentration of CNPs and scaffold@CNPs higher than 50 μg/mL can be used to inhibit the growth of P. aeruginosa. The fact that the scaffold@CNPs significantly reduced the expression of resistance genes, it has the potential to be used for medical applications such as wound dressings.

【 授权许可】

CC BY