Multi-antibiotics Resistant Relatedness of bla-gene Encoded Enteric Bacteria harbouring High Molecular R-plasmids.

8th International Biotechnology Conference, Exhibition and Workshop

Akinduti, P.A.^1 ; Oluwadun, A.^2 ; Osiyemi, J.^3 ; Ejilude, O.^2 ; Isibor, P.O.^1

Department of Biological Sciences, Covenant University, Ota, Ogun state, Nigeria^1

Department of Medical Microbiology and Parasitology, Olabisi Onabanjo University, Sagamu campus, Ogun state, Nigeria^2

Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria^3

关键词: Antibiotic resistance;    Antibiotic susceptibilities;    Cluster sampling;    High molecular weight;    Klebsiella oxytoca;    Pseudomonas aeruginosa;    R-Plasmid;    Resistant;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/210/1/012002/pdf
DOI  :  10.1088/1755-1315/210/1/012002

来源: IOP

【 摘 要 】

Increase prevalence of multi-resistant enteric bacteria isolates encoded with high mobile R-plasmid causing enteric infections was examined among the community residents in Abeokuta, Nigeria. Random cluster sampling of 251 fecal samples of community residents were cultured for enteric bacteria and biotyped. Disc diffusion and Micro-broth dilution assay were used to determine antibiotic susceptibility while R-plasmid was profiled with photo-gel documentation. Antibiotic resistance relatedness was detected using DendroUPGMA construction utility software. Of all isolates obtained, 31.3% were Escherichia coli Klebsiella oxytoca (19.5%), Pseudomonas aeruginosa (15.3%) and Shigella specie (2.0%). Significant high rate of 62.6% showed resistant to Cefuroxime, 61.6% to Ampicillin and Augmentin (54.2%) while 44.7%, 38.9% and 33.9% resist Cotrimoxazole, Ciprofloxacin and Tetracycline respectively at MIC ≥16 μg/ml (p = 0.004). Only 54.1% harboured high molecular weight R-plasmid (>11.0kbp) and 2.7% having