The Pseudomonas aeruginosa Type III Translocon Is Required for Biofilm Formation at the Epithelial Barrier

PLoS Pathogens

Michael Givskov¹  Alan R. Hauser¹  Henrik Almblad¹  Stephanie M. Rangel²  Joanne N. Engel²  Cindy S. Tran³  Arlinet Kierbel⁴  Tim Tolker-Nielsen⁵ 

[1] Costern Biofilm Center, Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark;Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois, United States of America;Department of Pediatrics, University of California San Francisco, San Francisco, California, United States of America;Institut Pasteur de Montevideo, Montevideo, Uruguay;Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore

关键词: Bacterial biofilms;    Pseudomonas aeruginosa;    Pulmonary imaging;    Antibiotic resistance;    Host cells;    Cell staining;    Confocal microscopy;    Mouse models;   

DOI  :  10.1371/journal.ppat.1004479

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Clinical infections by Pseudomonas aeruginosa, a deadly Gram-negative, opportunistic pathogen of immunocompromised hosts, often involve the formation of antibiotic-resistant biofilms. Although biofilm formation has been extensively studied in vitro on glass or plastic surfaces, much less is known about biofilm formation at the epithelial barrier. We have previously shown that when added to the apical surface of polarized epithelial cells, P. aeruginosa rapidly forms cell-associated aggregates within 60 minutes of infection. By confocal microscopy we now show that cell-associated aggregates exhibit key characteristics of biofilms, including the presence of extracellular matrix and increased resistance to antibiotics compared to planktonic bacteria. Using isogenic mutants in the type III secretion system, we found that the translocon, but not the effectors themselves, were required for cell-associated aggregation on the surface of polarized epithelial cells and at early time points in a murine model of acute pneumonia. In contrast, the translocon was not required for aggregation on abiotic surfaces, suggesting a novel function for the type III secretion system during cell-associated aggregation. Supernatants from epithelial cells infected with wild-type bacteria or from cells treated with the pore-forming toxin streptolysin O could rescue aggregate formation in a type III secretion mutant, indicating that cell-associated aggregation requires one or more host cell factors. Our results suggest a previously unappreciated function for the type III translocon in the formation of P. aeruginosa biofilms at the epithelial barrier and demonstrate that biofilms may form at early time points of infection.

【 授权许可】

CC BY