MSCs from polytrauma patients: preliminary comparative study with MSCs from elective-surgery patients

Stem Cell Research & Therapy

Gerardo J. Martí-Chillón¹  Silvia Preciado²  Sandra Muntión³  Fermín Sánchez-Guijo⁴  Carmen da Casa⁵  Javier González-Robledo⁶  Raúl López⁷  David Pescador⁸  Juan F. Blanco⁹ 

[1] Haematology Department, University Hospital of Salamanca, Salamanca, Spain;Haematology Department, University Hospital of Salamanca, Salamanca, Spain;Network Center in Regenerative Medicine and Cellular Therapy of Castilla y León, Salamanca, Spain;Haematology Department, University Hospital of Salamanca, Salamanca, Spain;TerCel Network, ISCIII, Madrid, Spain;Network Center in Regenerative Medicine and Cellular Therapy of Castilla y León, Salamanca, Spain;Haematology Department, University Hospital of Salamanca, Salamanca, Spain;Universidad de Salamanca (USAL), Salamanca, Spain;TerCel Network, ISCIII, Madrid, Spain;Network Center in Regenerative Medicine and Cellular Therapy of Castilla y León, Salamanca, Spain;Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain;Intensive Medicine Department, University Hospital of Salamanca, Salamanca, Spain;Orthopaedic Surgery and Traumatology Department, University Hospital of Salamanca, Salamanca, Spain;Orthopaedic Surgery and Traumatology Department, University Hospital of Salamanca, Salamanca, Spain;Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain;Orthopaedic Surgery and Traumatology Department, University Hospital of Salamanca, Salamanca, Spain;Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain;Universidad de Salamanca (USAL), Salamanca, Spain;TerCel Network, ISCIII, Madrid, Spain;

关键词: Polytrauma;    Bone regeneration;    Mesenchymal stromal cells;    Mesenchymal stem cells;    MSC;    Cellular therapy;   

DOI  :  10.1186/s13287-021-02500-9

来源: Springer

【 摘 要 】

BackgroundPolytrauma is a major clinical problem due to its impact on morbidity and mortality, especially among the younger population. Its pathophysiology is not completely elucidated, and the study of the involvement of certain cell populations with therapeutic potential, such as mesenchymal stromal cells (MSCs), is an area of growing interest, as mesenchymal cells have anti-inflammatory, immunoregulatory, and osteogenic potential.MethodsIn the present preliminary work, we have evaluated the characteristics of MSCs in terms of proliferation, immunophenotype, cell cycle, clonogenic capacity, and multilineage differentiation ability in a series of 18 patients with polytrauma and compared them to those from otherwise healthy patients undergoing elective spinal surgery.ResultsMSCs from polytrauma patients displayed higher proliferative potential with significantly higher cumulative population doublings, increased expression of some important cell adhesion molecules (CD105, CD166), and an early pre-osteogenic differentiation ability compared to those of the control group.ConclusionsMSCs could potentially be of help in the repair process of polytrauma patients contribute to both cell-tissue repair and anti-inflammatory response. This potential should be further explored in larger studies.

【 授权许可】

CC BY