Generation of magnetic biohybrid microrobots based on MSC.sTRAIL for targeted stem cell delivery and treatment of cancer

Cancer Nanotechnology

Research

Nyasha Nago¹  Michael Hamblin¹  Kiana Abolfathi²  Ali Kafash Hoshiar²  Rebekah Anamarie Gundersen³  Phoebe Elizabeth Blair³  Tianyuan Chu³  Serap Gokcen Dogan³  Andrea Mohr³  Ralf Michael Zwacka³  Greg Nicholas Brooke⁴ 

[1] Haematology Unit, East Suffolk and North Essex NHS Foundation Trust, CO4 5JL, Colchester, UK;School of Computer Science and Electronic Engineering, University of Essex, CO4 3SQ, Colchester, UK;School of Life Sciences, Protein Structure and Mechanism of Disease Group, Cancer and Stem Cell Biology Laboratory, University of Essex, CO4 3SQ, Colchester, UK;School of Life Sciences, Protein Structure and Mechanism of Disease Group, Molecular Oncology Laboratory, University of Essex, CO4 3SQ, Colchester, UK;

关键词: Biohybrid microrobots;    Mesenchymal stromal cells;    sTRAIL;    sPD1HAC;    Rotating magnetic field;    Mesenchymal stem cells;    Cancer;   

DOI  :  10.1186/s12645-023-00203-9

 received in 2023-03-14, accepted in 2023-04-25,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundCombining the power of magnetic guidance and the biological activities of stem cells transformed into biohybrid microrobots holds great promise for the treatment of several diseases including cancer.ResultsWe found that human MSCs can be readily loaded with magnetic particles and that the resulting biohybrid microrobots could be guided by a rotating magnetic field. Rotating magnetic fields have the potential to be applied in the human setting and steer therapeutic stem cells to the desired sites of action in the body. We could demonstrate that the required loading of magnetic particles into stem cells is compatible with their biological activities. We examined this issue with a particular focus on the expression and functionality of therapeutic genes inside of human MSC-based biohybrid microrobots. The loading with magnetic particles did not cause a loss of viability or apoptosis in the human MSCs nor did it impact on the therapeutic gene expression from the cells. Furthermore, the therapeutic effect of the gene products was not affected, and the cells also did not lose their migration potential.ConclusionThese results demonstrate that the fabrication of guidable MSC-based biohybrid microrobots is compatible with their biological and therapeutic functions. Thus, MSC-based biohybrid microrobots represent a novel way of delivering gene therapies to tumours as well as in the context of other diseases.

【 授权许可】

CC BY   
© The Author(s) 2023

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