Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway

Stem Cell Research & Therapy

Congying Zhao¹  Hao Zhang¹  Rong Huang¹  Xueyong Li¹  Jinqing Li¹  Zhixiao Lin¹  Zhanjun Lei¹  Bin Lin¹  Yuchen Dong¹  Yuheng Zhang¹ 

[1] Department of Plastic Surgery, Tangdu Hospital, Airforce Military Medical University, 710038, Xi’an, China;

关键词: Epidermal stem cell;    Micro/nanofiber scaffold;    Cell differentiation;    Wound healing;    Notch pathway;   

DOI  :  10.1186/s13287-021-02418-2

来源: Springer

【 摘 要 】

BackgroundEpidermal stem cells (EpSCs) play a vital role in wound healing and skin renewal. Although biomaterial scaffolds have been used for transplantation of EpSCs in wound healing, the ex vivo differentiation of EpSCs limits their application.MethodsTo inhibit the differentiation of EpSCs and maintain their stemness, we developed an electrospun polycaprolactone (PCL)+cellulose acetate (CA) micro/nanofiber for the culture and transplantation of EpSCs. The modulation effect on EpSCs of the scaffold and the underlying mechanism were explored. Liquid chromatography-tandem mass spectrometry for label-free quantitative proteomics was used to analyze proteomic changes in EpSCs cultured on scaffolds. In addition, the role of transplanted undifferentiated EpSCs in wound healing was also studied.ResultsIn this study, we found that the PCL+CA micro/nanofiber scaffold can inhibit the differentiation of EpSCs through YAP activation-mediated inhibition of the Notch signaling pathway. Significantly differentially expressed proteomics was observed in EpSCs cultured on scaffolds and IV collagen-coated culture dishes. Importantly, differential expression levels of ribosome-related proteins and metabolic pathway-related proteins were detected. Moreover, undifferentiated EpSCs transplanted with the PCL+CA scaffold can promote wound healing through the activation of the Notch signaling pathway in rat full-thickness skin defect models.ConclusionsOverall, our study demonstrated the role of the PCL+CA micro-nanofiber scaffold in maintaining the stemness of EpSCs for wound healing, which can be helpful for the development of EpSCs maintaining scaffolds and exploration of interactions between biomaterials and EpSCs.

【 授权许可】

CC BY