Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth

Stem Cell Research & Therapy

Wenjuan Wang¹  Luye Lv²  Jie Ma³  Yunping Zhu⁴  Ling Leng⁵  Zhihong Wu⁵  Dunqin Gao⁵ 

[1] Department of Dermatology, Chinese PLA General Hospital, Beijing, China;Institute of NBC Defense, Beijing, China;State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Life Omics, Beijing, China;State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Life Omics, Beijing, China;Basic Medical School, Anhui Medical University, Hefei, Anhui, China;Stem Cell and Regenerative Medicine Lab, Department of Medical Science Research Center, Translational Medicine Center, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China;

关键词: Epidermal stem cell;    Extracellular vesicle;    Organoids;    Proteomics;    Epidermal development;    Extracellular matrix;   

DOI  :  10.1186/s13287-020-01933-y

来源: Springer

【 摘 要 】

Millions suffer from skin diseases. Functional interfollicular epidermal stem cells are needed in skin therapy or drug screening in vitro. We obtained functional interfollicular epidermal stem cells with intact stemness and cell junctions by treating them with Wnt3a. Moreover, epidermal stem cell-derived extracellular vesicles were useful in epidermal cell growth. Finally, functional epidermal 3D organoids with polarity were cultured using Wnt3a and the supernatant derived from interfollicular epidermal stem cells and fresh medium in a 1:1 ratio. These results provide novel directions for the improvement of skin organoids and their potential in clinical application.

【 授权许可】

CC BY