Cultivation of mammals early male germ cells in a semi liquid medium

International Scientific Conference «AGRITECH-2019: Agribusiness, Environmental Engineering and Biotechnologies»

农业科学;生态环境科学;生物科学

Vasileva, S.A.^1

Federal State Budget Scientific Institution Federal Scientific Center VIEV, Moscow, Russia^1

关键词: Cell differentiation;    Complex Processes;    Culture systems;    Extracellular matrices;    Microenvironments;    Seminiferous tubules;    Three dimensional matrices;    Three-dimensional structure;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/315/7/072014/pdf
DOI  :  10.1088/1755-1315/315/7/072014

来源: IOP

【 摘 要 】

Spermatogenesis is one of the most complex processes of cell differentiation. The ability to cultivate early male germ cells and subject them to differentiation to produce mature gametes in vitro is an important task for both biology and veterinary and regenerative medicine. In vivo, spermatogenesis is known to occur in a three-dimensional structure, in the convoluted seminiferous tubules of the male reproductive glands, and Sertoli cells and the extracellular matrix have a significant impact on the survival, proliferation and differentiation of early germ cells. At present, methods of creating a microenvironment, similar to the native, are being actively developed to support spermatogenesis in vitro. This paper reflects research on the use of semi-fluid media as a three-dimensional matrix for the cultivation of early male gametes of mammals. These culture systems have been shown to support the differentiation of early germ cells to the stage of elongated spermatids with the subsequent formation of spermatozoa. However, it is necessary to continue research to create a culture system that can induce and maintain complete spermatogenesis.