High Concentration of Benzyladenine Solution Stimulates Anthers for Inducing Callus in Ricinus Communis L.

5th Annual International Conference on Material Science and Engineering

Liu, Ying^1 ; Yan, Shuying^3 ; Yang, Fuguang^3 ; Li, Dongliang^3 ; Tang, Jianian^3 ; Liu, Guoxuan^3 ; Lin, Shiwan^3 ; Niu, Sufang^2 ; Yang, Yali^3

Faculty of Agricultural Science, Guang Dong Ocean University, Haida Road #1, Mazhang District, Zhanjiang, Guangdong

524088, China^1

Fisheries College, Guang Dong Ocean University, Zhanjiang

524088, China^2

Department of Basic Medicine, School of Medicine, Jiaying University, Meizhou, Guangdong, China^3

关键词: 6-benzylaminopurine;    Callus formation;    Callus induction;    Culture systems;    High frequency HF;    Murashige and Skoog mediums;    Ricinus communis;    Sodium nitroprusside;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/275/1/012011/pdf
DOI  :  10.1088/1757-899X/275/1/012011

来源: IOP

【 摘 要 】

An high-frequency protocol for induction of callus from anther explants of Ricinus communis was described. When anther explants of R. communis was cultured directly onto medium containing 6-benzylaminopurine (BA) induced formation of only poor quality callus that had a low induction frequency of anther callus (10.67%). However, treating the anther explants with high concentrations (7.5-120 mg/L) of BA solution for short time periods (5-80 min) helped to improve the induction frequency and enhance the quality of the callus formation significantly. The best callus induction (41.25%) was observed when anther explants were treated with 15 mg/L BA solution for 10 min before being inoculated onto hormone-free Murashige and Skoog (MS) medium for 30 days. In order to further optimize the culture system, after treated with 15 mg/L BA for 10 min, anther explants were inoculated on the hormone-free MS medium contained concentrations of sodium nitroprusside (SNP). The results showed that SNP significantly promoted the response of callus induction, especially when 8 mg/L SNP was applied, the the highest percentage of callus induction (60.37%) were gained.