Somatic embryogenesis of East Kalimantan local upland rice varieties

1st International Conference on Tropical Studies and Its Application

Nurhasanah^1 ; Ramitha^1 ; Supriyanto, B.^1 ; Sunaryo, W.^1

Department of Agroecotechnology, Faculty of Agriculture, Mulawarman University, Kampus Gunung Kelua, Jl. Pasir Balengkong No.1, East Kalimantan, Samarinda

75119, Indonesia^1

关键词: 6-benzylaminopurine;    Callus formation;    Callus induction;    Embryogenic callus;    Growth and development;    Plant propagation;    Somatic embryo;    Somatic embryogenesis;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/144/1/012031/pdf
DOI  :  10.1088/1755-1315/144/1/012031

来源: IOP

【 摘 要 】

Somatic embryogenesis is the formation, growth and development of embryos from somatic cells. Somatic embryo induction is one of the in vitro plant propagation techniques that is very important for plant developmental purposes. Four local upland rice varieties of East Kalimantan, Mayas Pancing, Gedagai, Siam and Serai, were used in this study. A total of 200 explants (mature rice grains) for each varieties were inoculated on MS solid medium supplemented with 1 mg L-1 2,4 Dichlorophenoxy acetic acid (2,4-D) and 0.5 mg L-1 6-Benzylaminopurine (BAP). The results showed that response of each variety differed to embryosomatic induction, indicated by callus induction rate and callus quality, in terms of callus color and structure. The fastest callus formation was sobserved in Gedagai variety (8 days) while Mayas Pancing (13 days) was the latest one. The rate of callus induction varied from 60 to 98.5 %, and Serai variety has the highest callus induction rate. The highest friable callus structure was found in Siam variety (89.1%) and the lowest was in Gedagai (62.5%). Callus color was dominated by the yellowish-white (transparent) on all varieties tested. Most of the callus was potential as embryogenic callus characterized from the nodular and globular of friable callus structure and its yellowish-white color.