Utilization of local goat ovary waste from slaughterhouse as a material source for in vitro culture, conservation and freezing of oocyte cells

International Conference on Science and Technology for Environmental Protection

生态环境科学

Ciptadi, G.^1 ; Rahayu, S.^2 ; Putri, A.I.^2 ; Karima, H.N.^3 ; Budiarto, A.^1 ; Susiati^4 ; Putri, P.F.^4 ; Mudawamah^5

Faculty of Animal Science, Brawijaya University, Indonesia^1

Faculty of Matemathic and Life Science-Biology, Brawijaya University, Indonesia^2

Life Sciences Laboratory, Brawijaya University, Indonesia^3

Institute of Biosains, Brawijaya University, Malang, Indonesia^4

Faculty of Animal Science, Universitas Islam Malang (UNISMA), Indonesia^5

关键词: Culture in vitro;    Culture systems;    Genetic materials;    Immature oocytes;    Material sources;    Research development;    research methods;    Standard deviation;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/259/1/012007/pdf
DOI  :  10.1088/1755-1315/259/1/012007

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

These study purposes are to analyse the potential and benefits of utilization local goat ovary waste obtained at slaughterhouses as a source of cells or genetic material for the purposes of research, conservation, and freezing of genetic material of immature and mature oocyte in vitro. Preantral and antral follicles were selected and isolated for an experimental In Vitro Maturation (IVM) and Growth (IVG) of immature small size oocytes and goat antral and preantral follicles. The research method is explorative using a case research about the potential supply of follicle cells and immature oocytes sources of a local goat. Ovaries obtained from selected goat from the slaughterhouse are isolated for follicles and immature oocytes for culture in vitro and cell freezing. Data obtained are presented in the mean value and standard deviation and analyzed descriptively. Each ovary for obtaining follicle and immature oocyte was selected and isolated using 2 different methods of slicing and aspiration. The result showed that the great potential of the material genetic source of local goat from the different condition of goat slaughtered in the different potential of the follicle (IVG) and immature oocyte isolated (IVM) for each ovary (3 to 6 follicles) and (4 to 8 oocytes) respectively. The result is considered as a high variation because of different condition and variation of ages from prepubertal goat to adult goat of more 3 years of ages. A number of the follicle and immature oocytes may be isolated of about 200 per day. The IVG of follicle resulted in a lower result of M-II.oocytes of about 10 % oocyte recovered, meanwhile IVM of immature small oocyte resulted of about 40 to 60 % maturation (IVM) base on the cumulus expansion and first polar body extrusion. Culture system IVG may provide not only of how producing a number of competence mature oocytes but also of investigating the physiology of follicular development and ovulation of Indonesian local goat, it's may produce many matured oocytes (M-II) in the possibility of high numbers for research development and genetic material conservation. It was concluded that both IVG and IVM methods may possible to utilize ovary waste as an important source of genetic material for research development cell culture and cell conservation.