Patients with a high proportion of immature and meiotically resistant oocytes experience defective nuclear oocyte maturation patterns and impaired pregnancy outcomes

REPRODUCTIVE BIOMEDICINE ONLINE

卷:36

Article

Lu, Yuechao¹  Ferrer-Buitrago, Minerva¹  Popovic, Mina¹  Neupane, Jitesh¹  De Vos, Winnok H.^2,3  Lierman, Sylvie¹  Van den Abbeel, Etienne¹  Van der Jeught, Margot¹  Nikiforaki, Dimitra¹  De Sutter, Petra¹  Heindryckx, Bjorn¹ 

[1] Ghent Univ Hosp, Dept Reprod Med, Ghent Fertil & Stem Cell Team G FaST, B-9000 Ghent, Belgium

[2] Univ Ghent, Fac Biosci Engn, Dept Mol Biotechnol, Cell Syst & Imaging Res Grp CSI, B-9000 Ghent, Belgium

[3] Univ Antwerp, Dept Vet Sci, Lab Cell Biol & Histol, B-2020 Antwerp, Belgium

关键词: Ca2+ signalling;    Chromosome misalignment;    Immature oocytes;    Meiosis resistant;    Nuclear maturation;    Spindle abnormality;   

DOI  :  10.1016/j.rbmo.2017.12.021

来源: Elsevier

【 摘 要 】

Patients presenting with abnormally high numbers of immature oocytes at retrieval are more likely to exhibit maturation resistant oocytes. However, the clinical relevance of such events remains unknown. We investigated nuclear maturation competence of immature oocytes from patients showing >40% of collected immature oocytes (Study group) and Controls, in which a normal number of mature oocytes (>= 60%) was retrieved. Following in-vitro culture, oocytes were classified as maturation resistant or in-vitro matured (IVM). Treatment outcomes were evaluated in Study and Control groups based on presence of maturation resistant oocytes. Overall, similarly high spindle and chromosome abnormality rates were observed in maturation resistant oocytes from both Study and Control groups. IVM oocytes from the Study group revealed significantly higher percentages of misaligned chromosomes compared with Controls (P < 0.05). Remarkably, Study group patients with at least one maturation resistant oocyte showed significantly reduced cumulative pregnancy and live birth rates compared with Control group maturation resistant patients (P < 0.05). When further investigating the aetiology, a maturation resistant mouse model revealed defective Ca2+ signalling of maturation resistant oocytes at germinal vesicular breakdown and parthenogenetic activation. In conclusion, appropriate treatment strategies, including clinical utilization of IVM oocytes from Study group patients, warrant further investigation. (c) 2018 Published by Elsevier Ltd on behalf of Reproductive Healthcare Ltd.

【 授权许可】

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