Stretchable cell culture platforms using micropneumatic actuators

Micro & nano letters

article

Yun Jung Heo¹  Tetsuo Kan²  Eiji Iwase³  Kiyoshi Matsumoto²  Isao Shimoyama² 

[1] Graduate School of Engineering, Tokyo University of Agriculture and Technology;Graduate School of Information Science and Technology, The University of Tokyo;School of Fundamental Science and Engineering, Waseda University

关键词: biochemistry;    biocontrol;    biomembrane transport;    bioMEMS;    calcium;    diaphragms;    mechanoception;    microactuators;    pneumatic actuators;    polymers;    soft lithography;    microfabrication;    stretchable cell culture platforms;    micropneumatic actuators;    elastic micropneumatic actuators;    soft lithography;    polydimethylsiloxane;    mechanical stimulus;    cell membrane stretching;    design flexibility;    intracellular calcium ion concentration;    cellular mechanotransduction;    PDMS diaphragm;    size 558 mum to 24.8 mum;    Ca;   

DOI  :  10.1049/mnl.2013.0476

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Background and objectives: Various intrathecal adjuvants have been clinically tried for the prolongation of intraoperative and postoperative analgesia. This study aims at evaluating the effects of intrathecal nalbuphine and clonidine as adjuvants to isobaric levobupivacaine in subarachnoid block. Methodology: 60 patients scheduled for elective infra umbilical surgeries were allocated into two groups of thirty each to receive 15 mg of 0.5% isobaric Levobupivacaine with either 1 mg nalbuphine (Group LN) or 30 µg clonidine (Group LC) intrathecally. Characteristics of spinal anesthesia in terms of sensory analgesia and motor blockade were noted. Hemodynamic parameters and adverse effects if any were recorded. Data obtained was compiled and statistically analysed with appropriate tests. Results: Onset of sensory and motor blocks was faster in group LN (2.43 ± 0.93 and 2.2 ± 0.9 min) compared to group LC (3.26 ± 1.04 and 3.13 ± 1.0 min). However, time to two segment regression (186.8 ± 24.5 vs 146.5 ± 21.4), total duration of effective analgesia (384.1 ± 56.6 vs 292.1 ± 40.9) and total duration of motor block (345.3 ± 41.7 vs 235.6 ± 29.5 min) were significantly prolonged in group LC than in group LN. There was no significant difference in hemodynamic changes and adverse effects between the groups. Conclusion: The addition of 30 µg clonidine to intrathecal 0.5% isobaric levobupivacaine as adjuvant, is associated with prolonged sensory and motor blockade with better perioperative analgesia compared to 1 mg nalbuphine.

【 授权许可】

CC BY|CC BY-ND|CC BY-NC|CC BY-NC-ND