Active mixing of immobilised enzymatic system in microfluidic chip

Micro & nano letters

article

Kirill A. Lukyanenko¹  Kirill I. Belousov²  Ivan A. Denisov¹  Anton S. Yakimov¹  Elena N. Esimbekova¹  Anton S. Bukatin¹  Anatoly A. Evstrapov⁴  Peter I. Belobrov¹ 

[1] Siberian Federal University;ITMO University;Institute of Biophysics SB RAS;Institute for Analytical Instrumentation

关键词: enzymes;    bioMEMS;    numerical analysis;    biological techniques;    biochemistry;    molecular biophysics;    starch gel;    point-of-care devices;    electromechanical speaker membrane;    bioluminescent reaction;    numerical simulations;    reaction chamber geometry;    luciferase;    bienzyme system;    dried reagent mixing;    dried reagent dissolution;    microfluidic chip;    immobilised enzymatic system;   

DOI  :  10.1049/mnl.2016.0646

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Parameters for sample introduction, dried reagents dissolution and mixing with sample for bienzyme system NAD(H):FMN-oxidoreductase and luciferase immobilised in microfluidic chip were successfully determined. Numerical simulations of reaction chamber geometry, flavin mononucleotide (FMN) escape from starch gel and mixing options were conducted to achieve higher sensitivity of bioluminescent reaction. Results of numerical simulations were verified experimentally. The active mixer for dried reagents was made from an electromechanical speaker’s membrane which was connected to the input of the chip. Such a mixer provided better efficiency than a passive mixing, and it is simple enough for use in point-of-care devices with any systems based on immobilised enzymes in chips.

【 授权许可】

CC BY|CC BY-ND|CC BY-NC|CC BY-NC-ND