Reversible bonding by dimethyl-methylphenylmethoxy siloxane – based stamping technique for reusable poly(dimethylsiloxane) microfluidic chip

Micro & nano letters

article

Hong Ha Cao¹  Thi Hong Nhung Dinh¹  Feriel S. Hamdi¹  Magdalèna Couty¹  Emile Martincic¹  Marion Woytasik¹  Elisabeth Dufour-Gergam¹ 

[1] Institut d'Electronique Fondamentale, Université Paris-Sud;SATIE, Ecole Normale Supérieure de Cachan, CNRS

关键词: microfluidics;    polymers;    packaging;    microfabrication;    silicones;    coatings;    adhesives;    curing;    cellular biophysics;    toxicology;    biological techniques;    bioMEMS;    reversible bonding;    dimethyl-methylphenylmethoxy siloxane based stamping technique;    reusable poly(dimethylsiloxane) microfluidic chip;    reversible packaging;    chip bottom substrate;    microfabrication process;    poly(dimethylsiloxane) PDMS-glass reversible bonding;    stamping technique;    silicone conformal coating;    adhesive layer;    PDMS channel;    spin-coating;    thermal curing;    high working flow rate-pressure;    cycle pealing bonding;    MTT cell proliferation assay;    noncytotoxicity;    DMPMS-stamping bonding technique;    PDMS biochips;    functionalised surfaces;   

DOI  :  10.1049/mnl.2014.0581

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Reversible packaging is very desirable for microfluidic chips: it allows changing the chip upper cap when it is damaged, cleaning and reusing the chip bottom substrate. This latter case becomes even more attractive when integrated components are present on the substrate and have required a complex and expensive microfabrication process. The feasibility of poly(dimethylsiloxane) (PDMS)/PDMS and PDMS/glass reversible bonding is demonstrated using the stamping technique. Dimethyl-methylphenylmethoxy siloxane (DMPMS), a type of silicone conformal coating, is used as an adhesive layer between the PDMS channel and the substrate (PDMS or glass). This technique is easy to perform as it only needs spin-coating and thermal curing steps. The bond strength is suitable for high working flow rate/pressure of liquid in the channel (up to 500 μl/min and 200 kPa). The cycle ‘pealing/bonding’ of the cap can be repeated up to five times. In addition, an MTT cell proliferation assay has been performed and suggests the non-cytotoxicity of DMPMS. Thus, the DMPMS-stamping bonding technique opens new perspectives for PDMS biochips where plasma treatment is not possible such as functionalised surfaces.

【 授权许可】

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