Flow-lysometry for cytoplasmic analysis of single cells in large populations

Micro & nano letters

article

Won Chul Lee¹  Albert P. Pisano²  Young-Ho Cho³  Frans A. Kuypers¹ 

[1] Children's Hospital Oakland Research Institute;Berkeley Sensor and Actuator Center, University of California at Berkeley;Cell Bench Research Center, Korea Advanced Institute of Science and Technology

关键词: biological techniques;    bioMEMS;    blood;    cellular biophysics;    flow measurement;    microchannel flow;    flow lysometry;    cytoplasmic analysis;    single cells;    cell detection;    cell lysis;    component sensing;    microfluidic channel;    red blood cells;    biological techniques;    bioMEMS;    blood;    cellular biophysics;    flow measurement;    microchannel flow;    flow lysometry;    cytoplasmic analysis;    single cells;    cell detection;    cell lysis;    component sensing;    microfluidic channel;    red blood cells;   

DOI  :  10.1049/mnl.2012.0722

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Nowadays, the Web is a common tool for students searching information about the subjects taught in the different university courses. Although this is a good tool for the first rapid knowledge, a deeper study is usually demanded. After many years of teaching a course about ceramic and composite materials in the Integrated Master in Mechanical Engineering of Faculty of Engineering of University of Porto, Portugal, the authors used the Bologna reformulation of the mechanical engineering course to introduce new teaching methodologies based on a project based learning methodology. One of the main innovations is a practical work that comprises the study of a recent ceramic scientific paper, using all the actual available tools, elaboration of a scientific report, work presentation and participation in a debate. With this innovative teaching method the enrolment of the students was enhanced with a better knowledge about the ceramics subject and the skills related with the CDIO competences. This paper presents the reasons for this implementation and explains the teaching methodology adopted as well as the changes obtained in the students’ final results.

【 授权许可】

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