Droplet-based protein chip with Ni–Co coated surface

Micro & nano letters

article

Chun-ping Zhang¹  Yaw-Jen Chang²  Zi-Huan Ding²  Cheng-Hao Chang² 

[1] School of Energy and Power Engineering, Huazhong University of Science and Technology;Department of Mechanical Engineering, Chung Yuan Christian University;Institute of Biomedical Technology (IBT), Chung Yuan Christian University

关键词: biochemistry;    bioMEMS;    cobalt alloys;    drops;    electrodeposition;    fluorescence;    lab-on-a-chip;    microfluidics;    nickel alloys;    proteins;    droplet-based protein chip;    Ni–Co coated surface;    immunoassays;    printed circuit board substrate;    microchannel layer;    electrodeposition;    histidine-tagged protein immobilisation;    droplet fusion;    mixing;    incubation;    fluorescence intensity;    droplet size;    NiCo;   

DOI  :  10.1049/mnl.2013.0376

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Pre-operative fasting aims to decrease the volume and increase the pH of gastric contents, hence reducing the risk of aspiration. According to the past literature gastric contents of 25 ml (0.4 ml/kg) and with pH ≤ 2.5 predisposes the patient to pulmonary aspiration hence pre-operative fasting was recommended. Use of two-dimensional ultrasonography is an accurate non-invasive tool to determine gastric volume. We compared the gastric volume using ultrasonography and pH of gastric aspirate by pH strip in patients after overnight fasting and after ingestion of 200 ml clear fluids (water) 2 h prior to surgery.

【 授权许可】

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