Molecular-based cross-species evaluation of bovine coronavirus infection in cattle, sheep and goats in Ghana

BMC Veterinary Research

Olivia Agbenyega¹  Christopher Antwi²  Yaw Adu-Sarkodie³  Jones Lamptey⁴  Richmond Yeboah⁴  Philip El-Duah⁵  Vitus Burimuah⁶  Eddie-Williams Owiredu⁷  Samuel Oppong⁸  Christian Drosten⁹  Yaw Oppong Frimpong^1,10  Michael Owusu^1,11  Augustina Sylverken^1,12  Ben Emikpe^1,13  Raphael Folitse^1,13  William Tasiame^1,14 

[1] Department of Agroforestry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Department of Animal Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana;Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana;Institute of Virology, Universitätsmedizin Berlin, Charite, Germany;Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana;Department of Molecular Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Department of Wildlife and Range Management, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Institute of Virology, Universitätsmedizin Berlin, Charite, Germany;Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana;Department of Animal Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana;Department of Medical Laboratory Technology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana;Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Institute of Virology, Universitätsmedizin Berlin, Charite, Germany;

关键词: Bovine coronavirus;    Cattle;    Sheep;    Goat;    Cross-species infection;   

DOI  :  10.1186/s12917-020-02606-x

来源: Springer

【 摘 要 】

BackgroundApart from the huge worldwide economic losses often occasioned by bovine coronavirus (BCoV) to the livestock industry, particularly with respect to cattle rearing, continuous surveillance of the virus in cattle and small ruminants is essential in monitoring variations in the virus that could enhance host switching. In this study, we collected rectal swabs from a total of 1,498 cattle, sheep and goats. BCoV detection was based on reverse transcriptase polymerase chain reaction. Sanger sequencing of the partial RNA-dependent RNA polymerase (RdRp) region for postive samples were done and nucleotide sequences were compared with homologous sequences from the GenBank.ResultsThe study reports a BCoV prevalence of 0.3%, consisting of 4 positive cases; 3 goats and 1 cattle. Less than 10% of all the animals sampled showed clinical signs such as diarrhea and respiratory distress except for high temperature which occurred in > 1000 of the animals. However, none of the 4 BCoV positive animals manifested any clinical signs of the infection at the time of sample collection. Bayesian majority-rule cladogram comparing partial and full length BCoV RdRp genes obtained in the study to data from the GenBank revealed that the sequences obtained from this study formed one large monophyletic group with those from different species and countries. The goat sequences were similar to each other and clustered within the same clade. No major variations were thus observed between our isolates and those from elsewhere.ConclusionsGiven that Ghana predominantly practices the extensive and semi-intensive systems of animal rearing, our study highlights the potential for spillover of BCoV to small ruminants in settings with mixed husbandry and limited separation between species.

【 授权许可】

CC BY